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DavidRLiu_2019-_我们能重写dna来治疗基因疾病吗?_-

The most important gift your mother and father ever gave you was the two sets of three billion letters of DNA that make up your genome . 你父母给你的最重要的礼物 就是2组包含30亿个碱基的DNA, 它们构成了你的基因组。
genome:n.基因组;染色体组;
But like anything with three billion components , that gift is fragile . 但就像任何包含太多零件的东西一样, 这个礼物非常脆弱。
components:n.部件;组件;成份(component复数); fragile:adj.脆的;易碎的;
Sunlight , smoking, unhealthy eating, even spontaneous mistakes made by your cells, all cause changes to your genome. 太阳光、吸烟、不健康的饮食, 甚至是细胞自身出现的错误, 都能改变你的基因组。
Sunlight:n.日光; unhealthy:不健康的 spontaneous:adj.自发的;自然的;无意识的;
The most common kind of change in DNA is the simple swap of one letter, or base, such as C, with a different letter, such as T, G or A. 最常见的DNA改变 就是一个字母,也叫一个碱基, 比如C(胞嘧啶), 换成了别的碱基,如T(胸腺嘧啶)、 G(鸟嘌呤)或者A(腺嘌呤)。
swap:n.交换;交换之物;vt.与...交换;以...作交换;vi.交换;交易;
In any day, the cells in your body will collectively accumulate billions of these single-letter swaps , which are also called "point mutations ." 每一天,你身体里的细胞 会累计发生数亿次 单碱基的改变, 这也被称作“点突变”。
collectively:adv.共同地,全体地; accumulate:vi.累积;积聚;vt.积攒; swaps:互惠信贷; mutations:n.[遗]突变;变化;转变(mutation的复数形式);
Now, most of these point mutations are harmless. 大部分点突变是无害的。
But every now and then , a point mutation disrupts an important capability in a cell or causes a cell to misbehave in harmful ways. 但时不时, 点突变会干扰细胞的某项重要功能, 或者引起细胞出现异常行为。
every now and then:不时地;常常; disrupts:v.使…紊乱;使…瓦解(disrupt的单三形式); capability:n.才能,能力;性能,容量; misbehave:v.作弊;行为不礼貌;
If that mutation were inherited from your parents or occurred early enough in your development, then the result would be that many or all of your cells contain this harmful mutation. 如果这种变异是从父母遗传而来的, 或者发生于你生命早期, 那么结果很可能是 你的大部分甚至全部细胞 都带有这种有害变异。
inherited:v.继承(金钱、财产等);接替(责任等);继任;(inherit的过去式和过去分词) occurred:v.发生;出现;存在于;出现在;(occur的过去分词和过去式)
And then you would be one of hundreds of millions of people with a genetic disease , such as sickle cell anemia or progeria or muscular dystrophy or Tay-Sachs disease. 你可能就会像其他成千上万人一样 患上基因疾病, 像镰刀型红血球病,或者早衰症, 或者肌肉萎缩症, 或者家族黑蒙性痴呆症。
genetic:adj.基因的;遗传学的; disease:n.病,[医]疾病;弊病;vt.传染;使…有病; sickle:n.镰刀; anemia:n.贫血;贫血症; progeria:早老症;早衰症;儿童早衰症; muscular dystrophy:n.肌营养不良症;肌肉萎缩; Tay-Sachs:n.家族黑蒙性白痴;adj.家族黑蒙性白痴病的;
Grievous genetic diseases caused by point mutations are especially frustrating , because we often know the exact single-letter change that causes the disease and, in theory, could cure the disease. 由点基因突变引起的 这些不幸的遗传疾病 让我们尤其沮丧, 因为我们往往已经知道 哪个具体字母(碱基)发生了突变, 从而导致了疾病。 因此理论上,我们可以治愈它。
Grievous:adj.痛苦的;剧烈的; diseases:n.[医]病(disease的复数);[医]疾病;[植保]病害;疾病种类; especially:adv.尤其;特别;格外;十分; frustrating:adj.令人沮丧的;v.使沮丧;(frustrate的现在分词)
Millions suffer from sickle cell anemia because they have a single A to T point mutations in both copies of their hemoglobin gene. 数百万人被镰刀型红血球病折磨, 因为他们的血红蛋白基因中 都含有从A到T的点突变。
hemoglobin:n.[生化]血红蛋白(等于haemoglobin);血红素;
And children with progeria are born with a T at a single position in their genome where you have a C, with the devastating consequence that these wonderful, bright kids age very rapidly and pass away by about age 14. 而患有早衰症的孩子 只不过生来就在基因组中的 某个位置有一个T, 而正常的基因应该是C, 令人悲伤的是,这些聪明美好的孩子 衰老得非常快,通常活不过14岁。
devastating:adj.毁灭性的; v.毁灭; (devastate的现在分词) consequence:n.结果;重要性;推论; pass away:去世;停止;度过时间;
Throughout the history of medicine, we have not had a way to efficiently correct point mutations in living systems, to change that disease-causing T back into a C. 纵观整个医药史, 我们还没有找到有效的方法 可以在生命系统中纠正点突变, 将引起疾病的T改回正常的C。
Throughout:adv.自始至终,到处;全部;prep.贯穿,遍及; efficiently:adv.有效地;效率高地(efficient的副词形式); disease-causing:致病的;
Perhaps until now. 但现在我们有办法了。
Because my laboratory recently succeeded in developing such a capability, which we call "base editing ." 因为我的实验室 最近成功发明了一种技术, 叫做“碱基编辑”。
laboratory:n.实验室,研究室; recently:adv.最近;新近; editing:v.编辑,校订(文章、书籍等);编选;(edit的现在分词)
The story of how we developed base editing actually begins three billion years ago. 关于我们如何发明“碱基编辑”的故事 可以追溯到30亿年前。
We think of bacteria as sources of infection , but bacteria themselves are also prone to being infected , in particular , by viruses. 我们通常认为细菌是感染源, 但其实细菌本身也容易被感染, 特别是被病毒。
bacteria:n.[微]细菌; sources:n.来源;出处;起源;根源;原因;v.(从…)获得(source的第三人称单数和复数) infection:n.感染;传染;(身体某部位的)感染;传染病; prone:adj.俯卧的;有…倾向的,易于…的; infected:adj.带菌的; v.传染; (infect的过去分词和过去式) in particular:尤其,特别;
So about three billion years ago, bacteria evolved a defense mechanism to fight viral infection. 因此大约30亿年前, 细菌进化出一种防御机制, 来抵抗病毒感染。
evolved:v.(使)逐渐形成;进化;进化形成;(evolve的过去分词和过去式) mechanism:n.机制;原理,途径;进程;机械装置;技巧; viral:adj.滤过性毒菌引起的;滤过性毒菌的;
That defense mechanism is now better known as CRISPR. 这种防御机制如今被称为CRISPR。
And the warhead in CRISPR is this purple protein that acts like molecular scissors to cut DNA, breaking the double helix into two pieces. CRISPR里最强的武器 是这种紫色的蛋白质, 它就像分子剪刀一样, 可以剪断DNA链, 将双螺旋结构剪成2条单螺旋链。
warhead:n.弹头; purple:n.紫色;紫袍;v.变紫;使成紫色;adj.紫色的;华丽的文辞; protein:n.蛋白质; molecular:adj.[化学]分子的;由分子组成的; scissors:n.剪刀;v.剪断;删除;(scissor的第三人称单数和复数) double helix:双螺旋;
If CRISPR couldn't distinguish between bacterial and viral DNA, it wouldn't be a very useful defense system. 如果CRISPR分不清 细菌和病毒的DNA, 这就不能算是一个好的防御系统。
distinguish:vt.区分;辨别;使杰出,使表现突出;vi.区别,区分;辨别; bacterial:adj.[微]细菌的;
But the most amazing feature of CRISPR is that the scissors can be programmed to search for, bind to and cut only a specific DNA sequence. 但CRISPR最神奇之处在于 剪刀可以被编辑, 特定的DNA片段。
bind:v.结合;装订;有约束力;过紧;n.捆绑;困境;讨厌的事情;植物的藤蔓; specific:adj.特殊的,特定的;明确的;详细的;[药]具有特效的;n.特性;细节;特效药;
So when a bacterium encounters a virus for the first time, it can store a small snippet of that virus's DNA for use as a program to direct the CRISPR scissors to cut that viral DNA sequence during a future infection. 所以当细菌首次遇到某个病毒时, 它会存储一小段病毒的DNA 以此来引导CRISPR的剪刀, 如果将来发生感染, 就剪断病毒的DNA链。
bacterium:n.[微]细菌;杆菌属; encounters:v.遭遇(encounter的第三人称单数);邂逅;n.遭遇战(encounter的复数);相见; snippet:n.小片;片断;不知天高地厚的年轻人;
Cutting a virus's DNA messes up the function of the cut viral gene, and therefore disrupts the virus's life cycle . 剪断病毒的DNA 会扰乱该病毒基因的表达功能, 从而中断病毒的生命。
messes:v.弄糟;弄乱;妨碍(mess的第三人称单数);n.杂乱,脏乱(mess的名词复数); life cycle:n.生命周期;寿命(产品等从开发到使用完毕的一段时间);
Remarkable researchers including Emmanuelle Charpentier, George Church, 许多优秀的研究者,比如 埃马纽埃尔·卡彭蒂耶、乔治·丘奇,
Remarkable:adj.卓越的;非凡的;值得注意的;
Jennifer Doudna and Feng Zhang showed six years ago how CRISPR scissors could be programmed to cut DNA sequences of our choosing, including sequences in your genome, instead of the viral DNA sequences chosen by bacteria. 詹妮佛·杜德纳和张锋, 在6年前展示了 CRISPR的剪刀可以被编辑, 用来剪断我们选择的DNA片段, 人类的基因片段, 而不是细菌选的病毒的DNA片段。
sequences:n.[数][计]序列,顺序;继起的事(sequence的复数形式);
But the outcomes are actually similar. 效果是相似的。
outcomes:n.结果;成果;后果;出路;(outcome的复数)
Cutting a DNA sequence in your genome also disrupts the function of the cut gene, typically , by causing the insertion and deletion of random mixtures of DNA letters at the cut site . 通过剪断基因中的DNA片段 同样会影响被剪基因的功能, 方法就是在被剪的位置上增加或删除 随机的DNA碱基组合。
typically:adv.代表性地;作为特色地; insertion:n.插入;嵌入;插入物;(书、文章等中)添加的东西; deletion:n.删除;[遗]缺失;删除部分; random:adj.[数]随机的;任意的;胡乱的;n.随意;adv.胡乱地; mixtures:n.[化学]混合物(mixture的复数);配合料; site:n.地点;位置;场所;v.设置;为…选址;
Now, disrupting genes can be very useful for some applications. 在某些情况下,扰乱基因非常有用。
disrupting:v.扰乱;使中断;打乱;(disrupt的现在分词) genes:n.基因;(gene的复数)
But for most point mutations that cause genetic diseases, simply cutting the already-mutated gene won't benefit patients , because the function of the mutated gene needs to be restored , not further disrupted . 但对于大部分引起遗传疾病的 点突变而言, 仅仅剪断已经发生变异的基因, 对病人而言并没有意义, 因为这些变异基因的功能需要重置, 而不是进一步打乱。
patients:n.接受治疗者,病人;(patient的复数) mutated:突变;变成;经受突变; restored:adj.精力充沛的;精力恢复的;v.修复;恢复健康;(restore的过去分词和过去式) disrupted:破坏;使瓦解;使分裂;使中断;使陷于混乱(disrupt的过去分词形式);
So cutting this already-mutated hemoglobin gene that causes sickle cell anemia won't restore the ability of patients to make healthy red blood cells. 因此,把那些引起镰刀型贫血的, 已经变异的血红蛋白基因剪断, 并不能恢复病人的造血功能。
And while we can sometimes introduce new DNA sequences into cells to replace the DNA sequences surrounding a cut site, that process , unfortunately , doesn't work in most types of cells, and the disrupted gene outcomes still predominate . 有时候我们可以加入 一些新的DNA片段到细胞中, 替代被剪断区域周围的DNA链, 但可惜的是这一过程 对大部分细胞不起作用, 被影响的基因仍占主导地位。
process:v.处理;加工;列队行进;n.过程,进行;方法,adj.经过特殊加工(或处理)的; unfortunately:adv.不幸地; predominate:vt.支配,主宰;在…中占优势;vi.占主导(或支配)地位;占优势;
Like many scientists, I've dreamed of a future in which we might be able to treat or maybe even cure human genetic diseases. 像许多科学家一样, 我梦想着未来有一天, 我们可以治疗甚至治愈 人类遗传疾病。
treat:v.治疗;处理;招待;款待;n.款待;乐事;乐趣;
But I saw the lack of a way to fix point mutations, which cause most human genetic diseases, as a major problem standing in the way . 但我们缺乏修复点突变的方法, 而点突变是大部分 人类基因疾病的主因, 是我们需要解决的主要问题。
in the way:妨碍;挡道;
Being a chemist , I began working with my students to develop ways on performing chemistry directly on an individual DNA base, to truly fix, rather than disrupt, the mutations that cause genetic diseases. 我是一名化学家,我跟我的学生们 一起研究将化学反应 应用于单个DNA碱基上的方法, 从而真正修复,而不仅仅是 终止引起基因疾病的变异。
chemist:n.化学家;药剂师;药房;化学师; performing:adj.表演的;演奏的;v.做;执行;演出;运转(perform的现在分词) directly:adv.直接地;立即;马上;正好地;坦率地;conj.一…就; individual:n.个人;有个性的人;adj.单独的;个别的;
The results of our efforts are molecular machines called "base editors." 我们的成果就是分子机器, 叫做“碱基编辑器”。
Base editors use the programmable searching mechanism of CRISPR scissors, but instead of cutting the DNA, they directly convert one base to another base without disrupting the rest of the gene. 碱基编辑器使用的是 类似CRISPR剪刀的可编程搜索机制, 但与剪断DNA不同的是, 它们直接将一个碱基变成另一个, 而不会破坏基因的其他部分。
programmable:adj.[计]可编程的;可设计的; convert:v.转换; n.改变宗教(或信仰、观点)的人;
So if you think of naturally occurring CRISPR proteins as molecular scissors, you can think of base editors as pencils, capable of directly rewriting one DNA letter into another by actually rearranging the atoms of one DNA base to instead become a different base. 如果将CRISPR蛋白质 比作分子剪刀的话, 碱基编辑器就像铅笔, 它能直接改写DNA碱基, 通过重新排列DNA碱基上的原子, 而不是将它变成一个不同的碱基。
naturally:adv.自然地;自然而然地;轻而易举;天生地;大方地; occurring:n.事件;事故;事变; proteins:n.[生化]蛋白质(protein复数); capable:adj.能干的,能胜任的;有才华的; rearranging:v.重新安排;改变(位日期、地点);(rearrange的现在分词) atoms:n.[物]原子(atom的复数);
Now, base editors don't exist in nature. 碱基编辑器在大自然中并不存在。
In fact, we engineered the first base editor, shown here, from three separate proteins that don't even come from the same organism . 实际上,我们制造的 第一个碱基编辑器,如图所示, 是由3种独立的蛋白质组成, 它们甚至都不是来自同一个生物体。
first base:n.一垒; organism:n.生物;有机体;有机组织;(尤指)微生物;
We started by taking CRISPR scissors and disabling the ability to cut DNA while retaining its ability to search for and bind a target DNA sequence in a programmed manner. 我们首先抑制CRISPR剪刀 剪断DNA的功能, 并通过编程的方法,保持其搜索和锁定 目标DNA片段的能力。
disabling:v.使不能;使失去能力;致残疾(disable的ing形式); retaining:adj.留住的;定位的;使固定的;v.保留(retain的现在分词);
To those disabled CRISPR scissors, shown in blue, we attached a second protein in red, which performs a chemical reaction on the DNA base C, converting it into a base that behaves like T. 在功能被抑制的CRISPR剪刀上, 图中蓝色的部分, 我们加上了第2种蛋白质, 在这里用红色标出, 它会与DNA碱基C发生化学反应, 将其转换成与T行为相似的碱基。
attached:adj.依恋;v.重视;把…固定;(attach的过去分词和过去式) performs:v.执行;表演;演出;履行(perform的第三人称单数) chemical:n.化学制品,化学药品;adj.化学的; reaction:n.反应,感应;反动,复古;反作用; converting:v.(使)转变,转换,转化;可转变为;归附;(convert的现在分词) behaves:v.表现;表现得体;有礼貌;(behave的第三人称单数)
Third, we had to attach to the first two proteins the protein shown in purple, which protects the edited base from being removed by the cell. 第3步,我们将图片中 用紫色标出的蛋白质 加在前2种蛋白质上, 来保护被编辑过的碱基不被细胞移除。
edited:v.编辑,校订;编选;编集;(edit的过去分词和过去式)
The net result is an engineered three-part protein that for the first time allows us to convert Cs into Ts at specified locations in the genome. 最终结果就是制造出一个 由3部分组成的蛋白质, 这也是我们在史上首次 将基因组特定位置的 碱基C转换为T。
specified:v.指定;具体说明;逐一登记;列入清单;(specified是specify的过去分词) locations:n.地方;地点;位置;定位(location的复数)
But even at this point, our work was only half done. 但做到这一步, 我们的工作也仅仅完成了一半。
Because in order to be stable in cells, the two strands of a DNA double helix have to form base pairs. 因为为了保持细胞的稳定, DNA双螺旋结构中的两条链 必须形成碱基对。
stable:n.马厩;牛棚;adj.稳定的;牢固的;坚定的;vi.被关在马厩;赶入马房; strands:n.线; v.搁浅;
And because C only pairs with G, and T only pairs with A, simply changing a C to a T on one DNA strand creates a mismatch , a disagreement between the two DNA strands that the cell has to resolve by deciding which strand to replace. 因为C只能跟G配对, T只能跟A配对, 如果只是将一链上的碱基C变成T, 会造成DNA双螺旋的不匹配, 要解决这个问题, 细胞需要决定替换哪一条链。
mismatch:vt.使配错;n.错配;不协调; resolve:vt.决定; vi.解决; n.坚决;
We realized that we could further engineer this three-part protein to flag the nonedited strand as the one to be replaced by nicking that strand. 我们认识到可以改进 这个由3部分组成的蛋白质, 将未编辑的那条链标记为 要被切割掉。
nicking:n.基因巧合;血管局部缩窄;v.刻痕于;轻伤;攻击(nick的现在分词);
This little nick tricks the cell into replacing the nonedited G with an A as it remakes the nicked strand, thereby completing the conversion of what used to be a C-G base pair into a stable T-A base pair. 这个小缺口诱骗细胞 用A取代未编辑的G, 因为它重新生成了完整的单链, 这样就完成了C-G碱基对 到稳定的T-A碱基对的转变。
remakes:n.重做;重制物;vt.再制; nicked:v.在…上划刻痕;使有缺口;偷窃;逮捕;(nick的过去分词和过去式) thereby:adv.从而,因此;在那附近;在那方面; conversion:n.转换;变换;[金融]兑换;改变信仰;
After several years of hard work led by a former post doc in the lab, Alexis Komor, we succeeded in developing this first class of base editor, which converts Cs into Ts and Gs into As at targeted positions of our choosing. 在实验室前博士后Alexis Komor 领导的几年努力工作之后, 我们成功地开发了第一代碱基编辑器, 将指定位置的C都转变为T, G都转变为A。
converts:vt.使转变; vi.转变,变换; n.皈依者;
Among the more than 35,000 known disease-associated point mutations, the two kinds of mutations that this first base editor can reverse collectively account for about 14 percent or 5,000 or so pathogenic point mutations. 在3.5万多个已知的 与点突变有关的疾病中, 第一代碱基编辑器可以逆转的两种突变 总共占致病点突变的 14%或5000种左右。
reverse:n.反面; v.颠倒; adj.相反的; account for:对…负有责任;对…做出解释;说明…的原因;导致;(比例)占; pathogenic:adj.致病的;病原的;发病的(等于pathogenetic);
But correcting the largest fraction of disease-causing point mutations would require developing a second class of base editor, one that could convert As into Gs or Ts into Cs. 但是,纠正大部分致病点突变 需要开发第二代碱基编辑器, 一个可以将A都转变为G 或T都转变为C的工具。
fraction:n.分数;小部分;小数;少量; second class:adj.第二流的;次劣的;adv.坐二等车;作为第二类邮件;
Led by Nicole Gaudelli, a former post doc in the lab, we set out to develop this second class of base editor, which, in theory, could correct up to almost half of pathogenic point mutations, including that mutation that causes the rapid-aging disease progeria. 在实验室前博士后 Nicole Gaudelli的领导下, 我们着手开发了这个第二代碱基编辑器, 从理论上讲,这样可以 纠正近一半的致病点基因突变, 包括导致早衰症的突变。
rapid-aging:un.快速老化;加速老化;
We realized that we could borrow, once again, the targeting mechanism of CRISPR scissors to bring the new base editor to the right site in a genome. 我们意识到我们可以再次借助, CRISPR剪刀的靶向机制, 将新的碱基编辑器 带到基因组的正确位置。
But we quickly encountered an incredible problem; namely , there is no protein that's known to convert A into G or T into C in DNA. 但我们很快遇到了 一个棘手的难题; 具体来说,在DNA中没有 已知的蛋白质 可以将A转化成G 或者T转化成C。
encountered:v.遭遇,遇到;偶然碰到;意外地遇见;(encounter的过去分词和过去式) incredible:adj.难以置信的,惊人的; namely:adv.也就是;即是;换句话说;
Faced with such a serious stumbling block , most students would probably look for another project, if not another research advisor. 面对如此严重的困难险阻, 很多学生可能会寻找其他方案, 而不是咨询其他研究顾问。
stumbling block:障碍物;绊脚石;
(Laughter) (笑声)
But Nicole agreed to proceed with a plan that seemed wildly ambitious at the time. 但Nicole同意继续实施一项 当时看来雄心勃勃的计划。
proceed:n.收入;卖得金额;收益;v.继续;接着做;行进;前往; wildly:adv.野生地;野蛮地;狂暴地;鲁莽地; ambitious:adj.野心勃勃的;有雄心的;热望的;炫耀的;
Given the absence of a naturally occurring protein that performs the necessary chemistry, we decided we would evolve our own protein in the laboratory to convert A into a base that behaves like G, starting from a protein that performs related chemistry on RNA. 鉴于缺乏一种自然产生的蛋白质 来进行必要的化学反应, 我们决定在实验室里 进化我们自己的蛋白质 来把A转化成一个像G一样的碱基, 从一种对RNA进行相关 化学反应的蛋白质开始。
absence:n.没有;缺乏;缺席;不注意;
We set up a Darwinian survival-of-the-fittest selection system that explored tens of millions of protein variants and only allowed those rare variants that could perform the necessary chemistry to survive. 我们建立了达尔文适者生存选择体系, 探索了数千万种蛋白质变异, 只允许那些能够进行 必要化学反应的罕见变异存活下来。
Darwinian:adj.达尔文的;达尔文学说的;n.进化论者; survival-of-the-fittest:un.最适者生存;适应生存; selection:n.选择;选拔;挑选;被挑选的人(或物); explored:v.探索;勘探;考察;探险;(explore的过去式和过去分词) variants:n.[计]变体;变异型(variant的复数);
We ended up with a protein shown here, the first that can convert A in DNA into a base that resembles G. 我们最终得到了这里显示的蛋白质, 第一个能把DNA中的A 转化成类似G的碱基。
resembles:v.看起来像:显得像:像(resemble的第三人称单数)
And when we attached that protein to the disabled CRISPR scissors, shown in blue, we produced the second base editor, which converts As into Gs, and then uses the same strand-nicking strategy that we used in the first base editor 当我们把这个蛋白质连接到 受到抑制的CRISPR剪刀上, 这里用蓝色标示, 第二代碱基编辑器就诞生了, 可以把A转变为G, 然后使用第一代碱基编辑器中 同样的链切割策略
strategy:n.策略;行动计划;部署;战略;
to trick the cell into replacing the nonedited T with a C as it remakes that nicked strand, thereby completing the conversion of an A-T base pair to a G-C base pair. 诱骗细胞用C取代未编辑的T, 当它重新生成单链后, 就完成了A-T碱基对 到G-C碱基对的转变。
(Applause) (鼓掌)
Thank you. 谢谢。
(Applause) (鼓掌)
As an academic scientist in the US, 作为一个美国学术科学家,
academic:adj.学术的;理论的;学院的;n.大学生,大学教师;学者;
I'm not used to being interrupted by applause . 我还不是很习惯被掌声打断。
interrupted:v.插嘴; adj.被阻止的,中断的; (interrupt的过去分词和过去式) applause:n.欢呼,喝采;鼓掌欢迎;
(Laughter) (笑声)
We developed these first two classes of base editors only three years ago and one and a half years ago. 我们开发的这两代碱基编辑器 分别诞生于3年前和1年半前而已。
But even in that short time , base editing has become widely used by the biomedical research community . 但在这短短的时间里, 碱基编辑器已经被 生物医学团队广泛使用。
short time:adj.短暂的;快速的(shorttime的变形); biomedical:adj.生物医学的; community:n.社区;[生态]群落;共同体;团体;
Base editors have been sent more than 6,000 times at the request of more than 1,000 researchers around the globe. 碱基编辑器应全球超过 1000位研究者的请求 已经被发送到全球各地多达6千次。
A hundred scientific research papers have been published already, using base editors in organisms ranging from bacteria to plants to mice to primates . 目前发表的相关科研论文多达百篇, 包括了从细菌到植物, 从老鼠到灵长类动物的生物体中 使用的碱基编辑器。
scientific:adj.科学的,系统的; organisms:n.[生物]生物体(organism的复数);[生物]有机体; primates:n.灵长类;
While base editors are too new to have already entered human clinical trials, scientists have succeeded in achieving a critical milestone towards that goal by using base editors in animals to correct point mutations that cause human genetic diseases. 碱基编辑器还太新, 尚未进入人体临床试验, 科学家们已经在为之努力了, 他们成功使用动物的碱基编辑器 来纠正导致人类遗传疾病的点突变。
clinical:adj.临床的;诊所的; critical:adj.鉴定的;[核]临界的;批评的,爱挑剔的;危险的;决定性的;评论的; milestone:n.里程碑,划时代的事件;
For example, a collaborative team of scientists led by Luke Koblan and Jon Levy , two additional students in my lab, recently used a virus to deliver that second base editor into a mouse with progeria, changing that disease-causing T back into a C and reversing its consequences at the DNA, RNA and protein levels. 比如, 由Luke Koblan和Jon Levy领导 的一个科学家合作小组, 外加我们实验室的两个学生, 最近使用了一种病毒 将第二代碱基编辑器 植入患有早衰症的老鼠体内, 把致病的T变回C, 并在DNA、RNA和蛋白质层面上 逆转了其导致的后果。
collaborative:adj.合作的,协作的; Levy:n.征收;征兵,征税;vt.征收(税等);征集(兵等);vi.征税;征兵; additional:adj.附加的,额外的; reversing:v.颠倒;彻底转变;使完全相反;(reverse的现在分词) consequences:n.后果,结果;影响(consequence的复数);
Base editors have also been used in animals to reverse the consequence of tyrosinemia , beta thalassemia , muscular dystrophy, phenylketonuria , a congenital deafness and a type of cardiovascular disease -- 碱基编辑器也被用于动物身上 来逆转酪氨酸血症, 地中海贫血,肌营养不良, 苯丙酮尿症,某种先天性耳聋 和某种类型的心血管疾病——
tyrosinemia:n.酪氨酸血症;高酷氨酸血症; beta:n.贝它(希腊字母表的第二个字母); thalassemia:n.地中海贫血;库利氏贫血; phenylketonuria:n.[泌尿]苯丙酮尿;[泌尿]苯丙酮酸尿; congenital:adj.先天的,天生的;天赋的; deafness:n.聋;听力不佳; cardiovascular:adj.[解剖]心血管的;
in each case, by directly correcting a point mutation that causes or contributes to the disease. 在这些案例中,通过直接纠正 导致或者参与致病的点突变 就可以逆转病症。
contributes:[图情]投稿;[经]捐助;[经]捐献(contribute的第三人称单数);
In plants, base editors have been used to introduce individual single DNA letter changes that could lead to better crops. 在植物中,碱基编辑器已被用于 引入单个DNA字符的改变 以带来更好的收成。
And biologists have used base editors to probe the role of individual letters in genes associated with diseases such as cancer . 生物学家也使用了碱基编辑器 来探索单个碱基 在与癌症等疾病相关的基因中的作用。
biologists:n.生物学家(biologist的复数); probe:n.探针;调查;vi.调查;探测;vt.探查;用探针探测; associated:adj.有关联的; v.联想; (associate的过去分词和过去式) cancer:n.癌症;恶性肿瘤;
Two companies I cofounded, Beam Therapeutics and Pairwise Plants, are using base editing to treat human genetic diseases and to improve agriculture. 我联合创办的两家公司, Beam Therapeutics和Pairwise Plants, 正使用碱基编辑器治疗人类基因疾病 和改善农业。
Beam:n.光线; v.发射(电波); Therapeutics:n.疗法,治疗学; Pairwise:adj.成对发生的;adv.成对地;成双地; improve:v.改进;改善;
All of these applications of base editing have taken place in less than the past three years: on the historical timescale of science, the blink of an eye. 所有这些对碱基编辑的应用 都发生在不到三年的时间里: 在科学的历史尺度上, 这只是一眨眼的功夫。
historical:adj.历史的;史学的;基于史实的; timescale:时间量程;时标; blink:vt.眨眼;使…闪烁;vi.眨眼;闪烁;n.眨眼;瞬间;闪光;
Additional work lies ahead before base editing can realize its full potential to improve the lives of patients with genetic diseases. 在碱基编辑器 提升基因疾病病人的生命质量前, 我们仍有很多额外的工作要做。
potential:n.潜能;可能性;[电]电势;adj.潜在的;可能的;势的;
While many of these diseases are thought to be treatable by correcting the underlying mutation in even a modest fraction of cells in an organ, delivering molecular machines like base editors into cells in a human being can be challenging. 尽管许多这些疾病被认为是 只需要纠正器官中 很小一部分细胞 的潜在突变就能治疗的, 将分子机器(如碱基编辑器) 送入人体细胞 仍然富有挑战。
treatable:adj.能治疗的;好对付的;能处理的; underlying:adj.根本的; v.构成…的基础; (underlie的现在分词) modest:adj.些许的;谦虚的;
Co-opting nature's viruses to deliver base editors instead of the molecules that give you a cold is one of several promising delivery strategies that's been successfully used. 利用自然界的病毒来传递碱基编辑器, 而不是让你感冒的分子来做这个, 是几种已经成功实践的 有前景的传递策略之一。
Co-opting:由现会员选举;指派(co-opt的现在分词); molecules:n.[化学]分子,微粒;[化学]摩尔(molecule的复数); delivery:n.[贸易]交付;分娩;递送; strategies:n.策略;行动计划;部署;战略;(strategy的复数)
Continuing to develop new molecular machines that can make all of the remaining ways to convert one base pair to another base pair and that minimize unwanted editing at off-target locations in cells is very important. 继续研究开发新的分子机器, 找到其他的方法 将一个碱基对转变成 另一个碱基对, 并尽量减少细胞非目标位置上 不必要的编辑 是非常重要的。
minimize:v.使减少到最低限度;降低;贬低;使显得不重要; unwanted:adj.不需要的;有害的;讨厌的;空闲的; off-target:脱靶;偏离目标;
And engaging with other scientists, doctors, ethicists and governments to maximize the likelihood that base editing is applied thoughtfully , safely and ethically , remains a critical obligation . 与其他科学家、医生、 伦理学家和政府合作, 最大限度地提高 碱基编辑用于深思熟虑、 安全和合乎道德的可能性, 仍然是一项重要义务。
engaging:adj.有趣的; v.吸引住(注意力、兴趣); (engage的现在分词) ethicists:n.伦理学家; maximize:vt.取…最大值;对…极为重视;vi.尽可能广义地解释;达到最大值; likelihood:n.可能性,可能; applied:adj.应用的;实用的;v.应用;使用;申请,请求;(apply的过去分词和过去式) thoughtfully:adv.沉思地;体贴地,亲切地; ethically:adv.伦理上; obligation:n.义务;责任;职责;(已承诺的或法律等规定的)义务;
These challenges notwithstanding , if you had told me even just five years ago that researchers around the globe would be using laboratory-evolved molecular machines to directly convert an individual base pair to another base pair at a specified location in the human genome efficiently and with a minimum of other outcomes, 尽管有这些挑战, 如果你在五年前告诉我 全球的研究人员 将使用实验室发明的分子机器 来直接有效地把单个碱基对 转变成另一个碱基对, 放在特定的基因组位置, 而且不会产生其他结果,
notwithstanding:adv.尽管,仍然;prep.尽管,虽然;conj.虽然; minimum:n.最低限度;最小值;最少量;极小量;adj.最低的;最小的;最低限度的;
I would have asked you, "What science-fiction novel are you reading?" 我会反问你, “你是不是在读哪本科幻小说?”
novel:adj.新奇的;异常的;n.小说;
Thanks to a relentlessly dedicated group of students who were creative enough to engineer what we could design ourselves and brave enough to evolve what we couldn't, 感谢我们孜孜不倦的学生, 他们有惊人的创造力来设计工具, 使得我们可以改造自身, 并勇敢地去进化 原本无法进化出的特征,
relentlessly:adv.残酷地,无情地; dedicated:adj.献身的; v.把…奉献给; (dedicate的过去分词和过去式) creative:adj.创造性的;
base editing has begun to transform that science-fiction-like aspiration into an exciting new reality, one in which the most important gift we give our children may not only be three billion letters of DNA, but also the means to protect and repair them. 碱基编辑已经开始将 科幻小说般的渴望 转变成令人兴奋的现实, 我们给孩子们最重要的礼物 可能不再只是30亿DNA个碱基, 同时还有保护和修复它们的方法。
transform:v.使改变;使改观;使转换;n.[数]变换式;[化]反式; aspiration:n.渴望;抱负;送气;吸气;吸引术; repair:v.修理;修补;修缮;补救;n.修理;修补;修缮;
Thank you. 谢谢。
(Applause) (鼓掌)
Thank you. 谢谢。